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Journal: bioRxiv

Article Title: Optimising the use of Oxford nanopore sequencing technology for detection of Wolbachia bacterial endosymbionts in Anopheles mosquitoes

doi: 10.1101/2025.06.16.659891

Figure Lengend Snippet:

Article Snippet: The 16S Barcoding Kit library preparation method was also able to provide consistent microbiome profiles in which Wolbachia was the dominant species which is consistent with previous profiles using 16S rRNA Illumina amplicon sequencing .

Techniques: Sequencing

All bar graphs depict sequencing runs using two different barcoding kits: the Rapid PCR Barcoding Kit 24 V14 (runs 2-6) and the 16S Barcoding Kit 24 V14 (runs 7,8,9,11,12). Each run is represented by a vertical column. A ) Total Data generated (GB). B ) Percentage of passed vs failed bases called for each run. C ) Total number of reads obtained in each sequencing run. D ) Percentage of unclassified passed reads and unclassified passed bases for each run.

Journal: bioRxiv

Article Title: Optimising the use of Oxford nanopore sequencing technology for detection of Wolbachia bacterial endosymbionts in Anopheles mosquitoes

doi: 10.1101/2025.06.16.659891

Figure Lengend Snippet: All bar graphs depict sequencing runs using two different barcoding kits: the Rapid PCR Barcoding Kit 24 V14 (runs 2-6) and the 16S Barcoding Kit 24 V14 (runs 7,8,9,11,12). Each run is represented by a vertical column. A ) Total Data generated (GB). B ) Percentage of passed vs failed bases called for each run. C ) Total number of reads obtained in each sequencing run. D ) Percentage of unclassified passed reads and unclassified passed bases for each run.

Article Snippet: The 16S Barcoding Kit library preparation method was also able to provide consistent microbiome profiles in which Wolbachia was the dominant species which is consistent with previous profiles using 16S rRNA Illumina amplicon sequencing .

Techniques: Sequencing, Generated

Libraries prepared and sequenced using either the Rapid PCR Barcoding Kit 24 V14 (Rapid) or 16S Barcoding Kit 24 V14 (16S).

Journal: bioRxiv

Article Title: Optimising the use of Oxford nanopore sequencing technology for detection of Wolbachia bacterial endosymbionts in Anopheles mosquitoes

doi: 10.1101/2025.06.16.659891

Figure Lengend Snippet: Libraries prepared and sequenced using either the Rapid PCR Barcoding Kit 24 V14 (Rapid) or 16S Barcoding Kit 24 V14 (16S).

Article Snippet: The 16S Barcoding Kit library preparation method was also able to provide consistent microbiome profiles in which Wolbachia was the dominant species which is consistent with previous profiles using 16S rRNA Illumina amplicon sequencing .

Techniques: